mouse anti traf6  (Santa Cruz Biotechnology)

Journal: Animals : an Open Access Journal from MDPI

Article Title: Modulation of the Toll-like Receptor Pathway in Ovine Endometria During Early Pregnancy

doi: 10.3390/ani15070917

Figure Lengend Snippet: Antibodies used for Western blot.

Article Snippet: Mouse anti-TRAF6 monoclonal antibody , sc-8409 , Santa Cruz Biotechnology, Santa Cruz, CA, USA , 0.2 μg/mL.

Techniques: Western Blot

Journal: Animals : an Open Access Journal from MDPI

Article Title: Modulation of the Toll-like Receptor Pathway in Ovine Endometria During Early Pregnancy

doi: 10.3390/ani15070917

Figure Lengend Snippet: Relative expression values of ( A ) TLR2 , ( B ) TLR3 , ( C ) TLR4 , ( D ) TLR5 , ( E ) IRAK1 , ( F ) TRAF6 , and ( G ) MYD88 mRNA in the endometrium. Significant differences ( p < 0.05) are indicated by different letters (a, b, c, and d).

Article Snippet: Mouse anti-TRAF6 monoclonal antibody , sc-8409 , Santa Cruz Biotechnology, Santa Cruz, CA, USA , 0.2 μg/mL.

Techniques: Expressing

Journal: Animals : an Open Access Journal from MDPI

Article Title: Modulation of the Toll-like Receptor Pathway in Ovine Endometria During Early Pregnancy

doi: 10.3390/ani15070917

Figure Lengend Snippet: Expression of TLR2, TLR3, TLR4, TLR5, IRAK1, TRAF6, and MyD88 proteins in the endometrium. Significant differences ( p < 0.05) are indicated by different letters (a, b, c, and d).

Article Snippet: Mouse anti-TRAF6 monoclonal antibody , sc-8409 , Santa Cruz Biotechnology, Santa Cruz, CA, USA , 0.2 μg/mL.

Techniques: Expressing

Journal: Cell reports

Article Title: MicroRNA-19b exacerbates systemic sclerosis through promoting Th9 cells

doi: 10.1016/j.celrep.2024.114565

Figure Lengend Snippet:

Article Snippet: Anti-TRAF6 (D-10) Mouse , Santa Cruz Biotechnology , Cat#: sc-8409; RRID: AB_628391.

Techniques: Purification, Control, Virus, Plasmid Preparation, Expressing, Retroviral, Recombinant, Activation Assay, Modification, Infection, Transfection, Lysis, Extraction, Western Blot, Electron Microscopy, Cell Isolation, Staining, Mutagenesis, Immunoprecipitation, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Gene Expression, cDNA Synthesis, Sequencing, Software, Real-time Polymerase Chain Reaction

Journal: International Journal of Immunopathology and Pharmacology

Article Title: Corilagin relieves atherosclerosis via the toll-like receptor 4 signaling pathway in vascular smooth muscle cells

doi: 10.1177/03946320241254083

Figure Lengend Snippet: Primers of qRT-PCR.

Article Snippet: The primary antibodies used were rabbit anti-mouse TLR4 1:1000 (Cat No. 30400-1-AP, Proteintech, Beijing, China), rabbit anti-mouse MyD88 1:800 (Cat No. A0980, ABclonal), rabbit anti-mouse TIRAP 1:800 (Cat No. A12606, ABclonal), rabbit anti-mouse TRAF6 1:1000 (Cat No. 12809-1-AP, Proteintech), rabbit anti-mouse NEMO 1:1000 (Cat No. 18474-1-AP, Proteintech), rabbit anti-mouse p38MAPK 1:1000 (Cat No. 14064-1-AP, Proteintech), and rabbit anti-mouse IRF5 1:1000 (Cat No. 10547-1-AP, Proteintech).

Techniques:

Journal: International Journal of Immunopathology and Pharmacology

Article Title: Corilagin relieves atherosclerosis via the toll-like receptor 4 signaling pathway in vascular smooth muscle cells

doi: 10.1177/03946320241254083

Figure Lengend Snippet: Effect of corilagin on the TLR4 signaling pathway in MOVAS cells stimulated by ox-LDL. (a) mRNA expression of TLR4, TIRAP, MyD88, TRAF6, p38, NEMO and IRF5 was measured by qRT-PCR. * p < .05 compared with the control group, # p < .05 compared with the ox-LDL group, ** p < .05 compared with the aspirin group as determined by one-way ANOVA and subsequent Student–Newman–Keuls q -test ( n = 5). (B. C) Protein abundance was measured by western blotting. * p < .05 compared with the control group, # p < .05 compared with the ox-LDL group, ** p < .05 compared with the aspirin group, as determined by one-way ANOVA and subsequent Student–Newman–Keuls q -test ( n = 5). (D, E) Abundance of IL-6 and MCP-1 in cell culture supernatant was measured by ELISAs. * p < .05 compared with the control group, # p < .05 compared with the ox-LDL group, ** p < .05 compared with the aspirin group as determined by one-way ANOVA and subsequent Student–Newman–Keuls q -test ( n = 5). (F) Effect of corilagin on the MOVAS cells proliferation. * p < .05 compared with the control group, # p < .05 compared with the ox-LDL group determined by one-way ANOVA and subsequent Student–Newman–Keuls q -test ( n = 5). (G, H) apoptosis ratio of MOVAS were detected by Annexin V staining. No significant difference was found among four groups determined by one-way ANOVA and subsequent Student–Newman–Keuls q -test ( n = 5).

Article Snippet: The primary antibodies used were rabbit anti-mouse TLR4 1:1000 (Cat No. 30400-1-AP, Proteintech, Beijing, China), rabbit anti-mouse MyD88 1:800 (Cat No. A0980, ABclonal), rabbit anti-mouse TIRAP 1:800 (Cat No. A12606, ABclonal), rabbit anti-mouse TRAF6 1:1000 (Cat No. 12809-1-AP, Proteintech), rabbit anti-mouse NEMO 1:1000 (Cat No. 18474-1-AP, Proteintech), rabbit anti-mouse p38MAPK 1:1000 (Cat No. 14064-1-AP, Proteintech), and rabbit anti-mouse IRF5 1:1000 (Cat No. 10547-1-AP, Proteintech).

Techniques: Expressing, Quantitative RT-PCR, Control, Quantitative Proteomics, Western Blot, Cell Culture, Staining